Laboratory of Ryohei Yasuda

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  1. R.Yasuda (2006) Imaging spatiotemporal dynamics of neuronal signaling using fluorescence resonance energy transfer and fluorescence lifetime imaging microscopy Curr.Opin.Neurobiol. , 16,551–561
  2. K.Svoboda and R.Yasuda (2006) Principles of two-photon excitation microscopy and its applications to neuroscience Neuron 50, 823–839.
  3. V.Scheuss, R.Yasuda, A.Sobczyk and K.Svoboda (2006) Nonlinear [Ca2+] signaling in dendrites and spines caused by activity-dependent depression of Ca2+ extrusion. J. Neurosci. 26: 8183-8194.
  4. R.Yasuda, C.D.Harvey, H.Zhong, A.Sobczyk, L.van Aelst and K.Svoboda (2006) Super-sensitive Ras activation in dendrites and spines revealed by 2-photon fluorescence lifetime imaging. Nat. Neurosci. 9: 283-291.
  5. T.Pologruto, R.Yasuda, K.Svoboda (2004) Monitoring neural activity and [Ca2+] with genetically encoded Ca2+ indicators. J. Neurosci. 24:9572-9579.
  6. E.Nimchinsky, R.Yasuda, T.Oertner, K.Svoboda (2004) The number of glutamate receptors opened by synaptic stimulation in single hippocampal spines. J. Neurosci. 24: 2054-2064.
  7. R.Yasuda, E.A.Nimchinsky, V.Scheuss, T.A.Pologruto, T.G.Oertner, B.Sabatini, K.Svoboda (2004) Imaging calcium concentration dynamics in small neuronal compartment. Science's STKE pl5, 10.
  8. H.Itoh, A.Takahashi, K.Adachi, H.Noji, R.Yasuda, M.Yoshida, K.Kinosita,Jr. (2004) Mechanically driven ATP synthesis by F1-ATPase. Nature 427: 465-468.
    News and Views R.L.Cross (2004) Turning the ATP motor. Nature 427: 407-408
  9. R.Yasuda, B.L.Sabatini, K.Svoboda (2003) Plasticity of calcium channels in dendritic spines. Nat. Neurosci. 6: 948-955.
  10. K.Shimabukuro, R.Yasuda, E.Muneyuki, K.Y.Hara, K.Kinosita,Jr. and M.Yoshida (2003) Catalysis and rotation of F1 motor: cleavage of ATP at the catalytic site occurs in 1 ms before 40o substep rotation. Proc. Natl. Acad. Sci. USA 100: 14731-14736.
  11. R.Yasuda, T.Masaike, K.Adachi, H.Noji, H.Itoh, K.Kinosit,Jr. and M.Yoshida (2003) The ATP-waiting conformation of rotating F1-ATPase revealed by single-pair fluorescence resonance energy transfer. Proc. Natl. Acad. Sci. USA 100: 9314-9318.
  12. Y.Hirono-Hara, H.Noji, M.Nishiura, E.Muneyuki, K.Y.Hara, R.Yasuda, K.Kinosita, Jr., and M.Yoshida (2001) Pause and rotation of F1-ATPase during catalysis. Proc. Natl. Acad. Sci. USA 98 : 13649-13654.
  13. R.Yasuda, H.Noji, M.Yoshida, K.Kinosita,Jr. and H.Itoh (2001) Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase. Nature 410: 898-904.
    News and Views M.J.Schnitzer (2001) Doing a rotary two-step. Nature 410: 878-881
  14. H.Noji, D.Bald, R.Yasuda, H.Itoh, M.Yoshida, and K.Kinosita,Jr.(2001) Purine, but not pyrimidine, nucleotides support rotation of F1-ATPase. J. Biol. Chem. 276: 25480-25486.
  15. K.Adachi, R.Yasuda, H.Noji, H.Itoh, Y.Harada and M.Yoshida, K.Kinosita,Jr. (2000) Stepping rotation of F1-ATPase visualized through angle-resolved single-fluorophore imaging. Proc.Natl.Acad.Sci.USA. 97: 7243-7247.
  16. R.Yasuda, H.Noji, K.Adachi, T.Nishizaka, Y.Kato-Yamada, M.Yoshida and K.Kinosita, Jr. (2000) Rotation of ATP synthase. In Na/K-ATPase and Related ATPases. K.Taniguchi and S.Kaya,Editors, Elsevier,Amsterdam,pp.187–190.
  17. K.Kinosita,Jr., R.Yasuda , H.Noji, K.Adachi (2000) F1-ATPase: a highly efficient rotary ATP machine. Essays Biochem. 33:3-18.
  18. K.Kinosita, Jr., R.Yasuda, H.Noji, and K.Adachi (2000) A rotary molecular motor that can work at near 100% efficiency. Philos.Trans.R.Soc.Lond.B Biol.Sci. 355: 473-489.
  19. T.Masaike, N.Mitome, H.Noji, E.Muneyuki, R.Yasuda, K.Kinosita,Jr., and M.Yoshida (2000), Rotation of F(1)-ATPase and the hinge residues of the beta subunit.J.Exp.Biol. 203 Pt 1:1-8.
  20. K.Y.Hara, H.Noji, D.Bald, R.Yasuda, K.Kinosita, Jr., and M.Yoshida (2000) The role of the DELSEED motif of the beta subunit in rotation of F1-ATPase. J. Biol. Chem. 275(19):14260-14263.
  21. N.Sasaki, H.Asukagawa, R.Yasuda, T.Hiratsuka, K.Sutoh (1999) Deletion of the myopathy loop of Dictyostelium myosin II and its impact on motor functions. J. Biol. Chem. 274(53): 37840-37844.
  22. Y.Arai, R.Yasuda, K.Akashi, H.Miyata, K.Kinosita, Jr., Y.Harada, and H.Itoh (1999) Tying a molecular knot with optical tweezers. Nature. 399:446-448.
  23. Y.Kato-Yamada, H.Noji, R.Yasuda, K.Kinosita,Jr., and M.Yoshida (1998) Direct observation of the rotation of epsilon subunit in F1-ATPase. J. Biol. Chem. 273:19375-19377.
  24. R.Yasuda*, H.Noji*, K.Kinosita,Jr., and M.Yoshida (1998) F1-ATPase is a highly efficient motor that rotates with discrete 120-degree steps. Cell. 93:1117-1124. *Equal contribution [movie]
    News and Views H.C.Berg (1998) Keeping up with the F1-ATPase. Nature. 394:324-325.
  25. K.Kinosita,Jr., R.Yasuda, H. Noji, S. Ishiwata, and M. Yoshida (1998) F1-ATPase: a rotary motor made of a single molecule. Cell 93: 21-24.
  26. R.Yasuda, H.Noji, K.Kinosita,Jr., F.Motojima, and M.Yoshida (1997) ATP synthase is a rotary motor protein machinery: Demonstration of rotation of g subunit. J.Bioenerg.Biomemb. 29: 207-209.
  27. Y.Suzuki, R.Ohkura, S.Sugiura, R.Yasuda, K.Kinosita,Jr., M.Tanokura, and K. Sutoh (1997) Modulation of actin filament sliding by mutations of the SH2 cysteine in Dictyostelium Myosin II. Biochem. Biophys. Res. Com. 234:701-706.
  28. H.Noji*, R.Yasuda*, M. Yoshida, and K. Kinosita, Jr. (1997) Direct observation of the rotation of F1-ATPase. Nature 386:299-302. *Equal contribution [movie]
    News and Views S.M.Block (1997) Real engines of creation Nature. 386:299-302.
  29. R.Yasuda, H.Miyata, and K.Kinosita,Jr. (1996) Direct measurement of the torsional rigidity of single actin filaments. J. Mol. Biol. 262:227-236.
  30. H.Miyata, R.Yasuda, and K.Kinosita,Jr. (1996) Strength and lifetime of the bond between actin and skeltal muscle alpha-actinin studied with an optical trapping technique. Biochim. Biophys. Acta 1290 : 83-88.
  31. H.Miyata, K.Akashi, H.Yoshikawa, R.Yasuda, S.Nishiyama, and K.Kinosita,Jr. (1994) Ultra-micro mechanical measurements on biological supramolecular systems. In Optical methods in biomedical and environmental sciences. vol.3. H.Ohzu and S.Komatsu,editors. Elsevier,Amsterdam,pp.187-190.
  32. H.Miyata, H.Hakozaki, H.Yoshikawa, N.Suzuki, R.Yasuda, and K.Kinosita, Jr. (1994) An analysis of stepwise motion of an actin filament over glass-bound heavy meromyosin. In Optical methods in biomedical and environmental sciences. vol.3. H.Ohzu and S.Komatsu,editors. Elsevier,Amsterdam,pp.171-174.