www.duke.edu/~yzhuang

Mouse Genotyping Resources

Mouse Genes and Mutations:


Gene/Mutation Primer ID PCR product size (kb)
  common mutant wildtype mutant wildtype
E2AER bjE2Ar3 YZ-29 YZ-164 1.2 0.9
E47bm YZ-104 YZ-29 YZ-164 2.0 1.7
E2Agal YZ-177 YZ-24 E19 0.9 1.1

E2Aflox

(wt vs. flox)

YZ-104 YZ-150 YZ-164 2.0 1.8

E2Aflox

(flox vs. deleted)

YZ-198 PGKneo for E2Aflox for 0.5 (deleted) 1.2 (flox)
E2Adel E2Aflox for YZ-198 E19 0.5 1.1
E2AGFP YZ-104 YZ-29 YZ-164 2.0 1.7
E2AHF YZ-104 YZ-29 YZ-164 2.0 1.7
HEBko YZ-119 YZ-29 YZ-122 2.8 2.2
*HEBbm YZ-199 YZ-122   1.8 2.0

HEBflox

(wt vs. flox)

JW1 JW2   1.3 1.1

HEBflox

(flox vs. deleted)

JW1 JW2   0.5 (deleleted) 1.3 (flox)
Id3ko YZ-151 YZ-29 YZ-170 2.5 2.1
TCR Vb8.2hCD2 P1 P3 P2 1.1 1.8
LckCre Lck-p Mx3   0.8  
CD4Cre CD4Crep Mx3B   0.45  
Mx-Cretg MxCre5 MxCre3   0.5  
Id2hCD5 Id2ra hCD5f Id2f 1.2 1.5

*For HEBbm genotyping, digest with NcoI after PCR to detect mutant vs. wt alleles.


PCR Genotyping Protocol

Genotyping Primer Sequences

Quantitative PCR analysis of E2A deletion